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- W2373107229 abstract "Objective: To investigated the shape and ultrastructure of the neuron-like cells derived from human BMSC,thus to explore whether the neuron-like cells derived from human BMSC possessed the shape and ultrastructure of mature neurons or not.Methods: Human BMSC were separated from adult bone marrow with density gradient centrifugation and adherent culture,induced and differentiated into neuron-like cells with EGF/ bFGF/DMSO/BHA.The expression of CD34,CD44,CD45 and other antigen on human BMSC were detected by flow cytometer(FCM),Nissl body by toluidine blue staining,ultrastructure of the neuron-like cells by electron microscope.Results: Flow cytometry displayed cells were positive for CD44,showing a typical mesenchymal like immunophenotype,on the other hand,they were negative for CD45 and CD34 markers associated with hematopoietic cells.After induced 2w by EGF/ bFGF/DMSO/BHA,almost bulk of cells had morphological features typical of neurons,such as spherical shape and extending processes.Analysis confirmed that toluidine blue staining for Nissl body had been induced by EGF/ bFGF/DMSO/BHA.Using electron microscope,we could also see lots of flat,vesicular rough endoplasmic reticulum(RER),some free ribosome and neurofilament in the cytoplasm.Conclusion: Neuron-like cells derived from human BMSC can express not only the typical shape of mature neurons but also the ultrastructure of mature neurons." @default.
- W2373107229 created "2016-06-24" @default.
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- W2373107229 date "2009-01-01" @default.
- W2373107229 modified "2023-09-26" @default.
- W2373107229 title "Shape and ultrastructure of the neuron-like cells derived from human BMSC" @default.
- W2373107229 hasPublicationYear "2009" @default.
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