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- W2373137622 abstract "Objective To survey the expression in gastric cancer of a new member cyclin G2 (belonging to G typed cyclin family) and its influence on cell strain SGC 7901.Methods Forty case gastric cancer specimens were examined by immunohistochemical technology to observe cyclin G2 expression and analyze the positive rates and average gray value′s change by micro image analysis system;the pIRES G2 plasmid was transferred into SGC 7901 cell line through cation lipofectamine reagent;cell clones were chosen by G418;pick up cell clone and enlarge culture to test cell proliferative ability by MTT assay (thiazolyl blue method).Results ①The positive rates of cyclin G2 in non cancer tissue, differentiation and low differentiation type cancer were 90%,83 3% and 22 7% (P0.01).The average gray values of cyclin G2 in non cancer tissue,differentiation type and low differentiation type cancer were 166±13,191±16 and 219±13 (P0.01).② The average absorbency of clone cell obtained by stable transfection pIRES G2 at 570 nm was 1.70±0.21,the average absorbency of clone cell obtained by stable transfection pIRESneo at 570 nm was 2 1±0 4,there was statistical differential notability between them (P0.01).Conclusion The expression level of cyclin G2 was associated with the differentiation degree of gastric carcinoma,it may serve as a biological marker in the evaluation of the malignancy of tumor,it can inhibit SGC 7901 cell proliferation ability obviously." @default.
- W2373137622 created "2016-06-24" @default.
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- W2373137622 date "2005-01-01" @default.
- W2373137622 modified "2023-09-25" @default.
- W2373137622 title "Expression of cyclin G2 in gastric carcinoma tissue and study of its effect on in vitro proliferation of gastric carcinoma cell strain" @default.
- W2373137622 hasPublicationYear "2005" @default.
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