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- W2373142818 abstract "AIM: To discuss the protection of cistanchis glycosides on ethanol-induced damage in the primary hepatocyte of mice. METHODS: The primary hepatocytes were collected by perfusion in situ method.The cell livability was evaluated by MTT,fluorescence microscopy and flow cytometry were used to evaluate the cell apoptosis and necrosis,immunohistochemistry was used to detect the expression of bcl-2 and c-fos.RESULTS:After the primary hepatocytes were damaged by alcohol,the cell livability decreased,apoptosis and necrosis were obvious,the expression of bcl-2 was decreased while that of c-fos was increased.Cistanchis glycosides could increase the cell livability,ameliorate apoptosis and necrosis,increase the expression of bcl-2,and decrease the expression of c-fos in a dose-dependent manner.CONCLUSION: The protective effects of cistanchis glycosides on ethanol-induced liver damage in the primary hepatocytes is associated with increasing the expression of bcl-2,and decreasing the expression of c-fos;resulting less apoptosis,less necrosis,and increasing the cell livability." @default.
- W2373142818 created "2016-06-24" @default.
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- W2373142818 date "2011-01-01" @default.
- W2373142818 modified "2023-09-27" @default.
- W2373142818 title "Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice" @default.
- W2373142818 hasPublicationYear "2011" @default.
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