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- W2373144643 abstract "Objective To investigate the effects of Shenfu injection on expressions of endothelial protein C receptor (EPCR ) mRNA, protein and NF-B in human umbilical vein endothelial cells (HUVECs) incubated with lipopolysaccharide(LPS). Methods The EPCR mRNA, protein or phosphorylated protein of NF-B in HUVECs was detected by reverse transcription polymerase chain reaction (RT-PCR) or western bloting (WB) respectively. Results The expressions of EPCR mRNA and protein were significantly lower in LPS stimulation (24 h) (0.01) and in LPS stimulation (48 h) group (0.05) than those in normal control group. However, the expressions of EPCR mRNA and protein were significantly higher in Shenfu (12 or 24 h) groups than those in LPS stimulation (12 or 24 h ) group (0.01; 0.05). The expressions of NF- B protein were significantly higher in LPS stimulation (24 and 48 h) groups than those in normal control group (0.05 ); the expressions of NF-B protein were significantly lower in Shenfu treated (12, 24 h or 48 h) groups than that in LPS stimulation group (all 0.05). Conclusion The data suggested that LPS could induce the decrease of EPCR mRNA and protein expressions through increasing the expression of NF- B in HUVECs, and Shenfu injection could block the inhibitory action of LPS on EPCR." @default.
- W2373144643 created "2016-06-24" @default.
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- W2373144643 date "2010-01-01" @default.
- W2373144643 modified "2023-09-26" @default.
- W2373144643 title "Effects of Shenfu injection on expressions of EPCR mRNA, protein and NF-B protein in human umbilical vein endothelial cells induced by lipopolysaccharide" @default.
- W2373144643 hasPublicationYear "2010" @default.
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