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- W2373171353 abstract "Objective To construct two highly efficient prokaryotic expression vectors of eukaryotic L-methionine γ-lyase genes(MGL1 MGL2),induce their expression,purify,and compare their activities.Methods Two types of recombinant plasmids pGEX-4T-1-MGL1 and pGEX-4T-1-MGL2 were established by molecular cloning technique.Then the recombinant plasmids were transformed into E.coli Dh5ct to induce expression.Results Two types of highly efficient recombinant expression plasmids pGEX-4T-1-MGL1 and pGEX-4T-1-MGL2 were constructed successfully.Purity of metase of pGEX-4T-1-MGL1 was 82.5%,activity was 0.5052 IU/mg.Purity of metase of pGEX-4T-1-MGL2 was 81.4%,activity was 0.3052 IU/mg.Conclusion PGEX-4 T-1-MGL1 and pGEX-4 T-1-MGL2 can express L-methionine γ-lyase,and L-methionine γ-lyase of PGEX-4 T-1-MGL1 is purer and more active." @default.
- W2373171353 created "2016-06-24" @default.
- W2373171353 creator A5080877177 @default.
- W2373171353 date "2012-01-01" @default.
- W2373171353 modified "2023-09-23" @default.
- W2373171353 title "Expression and Activities of Two Prokaryotic Expression Vectors of Eukaryotic L-methionine γ-lyase Genes" @default.
- W2373171353 hasPublicationYear "2012" @default.
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