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- W2373180843 abstract "The water-soluble crude polysaccharide (APP) was isolated from fruit bodies of Auricularia polytricha by hot water extraction, ethanol precipitation, and deproteination with Sevage method. It was found that APP could markedly inhibit growth of Sarcoma 180 in mice. APPⅠ, Ⅱ and Ⅲ was separated from APP by ion-exchange on DEAE-Cellulose-52, and APPⅡ couldsignificantly exhibit the anti-tumor effect. APPⅡ was further purified by gel-filtration chromatography on Sephacryl S-300 columns and separated into APPⅡA and APPⅡB. It was confirmed that APPⅡA could significantly inhibit tumor growth. APPⅡA was characterized by thin-layer chromatography (TLC), agarose gel electrophoresis (AGE), high performance liquid chromatography (HPLC), and UV, IR and 1H-NMR spectroscopy. The results demonstrated that APPⅡA was homogeneous and its relative molecular weight was approximately 110,000Da. Monosaccharide analysis showed that APPⅡA was a heteropolysaccharide which was composed of xylose and mannose. The glycosidic linkages of APPⅡA was estimated to contains both α- and β- pyranoside form." @default.
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- W2373180843 date "2009-01-01" @default.
- W2373180843 modified "2023-09-23" @default.
- W2373180843 title "Isolation,purification and structural characterization of bioactive polysaccharide APPIIA from Auricularia polytricha" @default.
- W2373180843 hasPublicationYear "2009" @default.
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