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- W2373188051 abstract "Objective To construct the chimeric recombinant plasmid vector encoding C、E2 and NS3 antigens of HCV. Methods The specific primers were designed and synthesized to amplify the DNA fragments(PCR method) of C、E2 and NS3 antigen genes from the recombinant plasmids pHCVc, pBE2 and pGMXNS3 5 of HCV respectively. The length of the acquired fragments were 501 bp, 603 bp and 900 bp.After being digested by appropriate restriction endoenzymes(HindⅢ, BamHⅠ, EcoRⅠ and XhoⅠ), the generated fragments of C、 E2 and NS3 genes were cloned into pcDNA3 one by one according to their specific orientations.Then the recombinant plasmid was identified by DNA sequencing and digesting of endoenzymes. Results The fragments digested by endoenzymes were as large as the predicted results.DNA sequencing was the same with the sequence reported on the literatures. Conclusion The chimeric recombinant plasmid pcDNA3 C E2 NS3 was successfully constructed." @default.
- W2373188051 created "2016-06-24" @default.
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- W2373188051 date "2001-01-01" @default.
- W2373188051 modified "2023-09-25" @default.
- W2373188051 title "Construction of the Chimeric Recombinant Plasmid Vector Encoding C,E2,NS3 Antigens of Hepatitis C Virus" @default.
- W2373188051 hasPublicationYear "2001" @default.
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