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- W2373200616 abstract "Glutathione S-transferases(GSTs) play important roles in detoxifying insecticides and antioxidation.In order to explore the roles of Bombyx mori glutathione S-transferase gene(BmGSTd1) in detoxification and resistance to insecticides,we used real-time fluorescent quantitative PCR method to detect the transcription levels of BmGSTd1 in different tissues of the 5th instar larvae feeding on both normal mulberry leaves and sodium fluoride treated mulberry leaves.The detection results were normalized by using 3 Bombyx mori internal reference genes namely Actin3,GAPDH and 28S rRNA.The results indicated that the transcriptional levels of BmGSTd1 were different in various tissues of the 5th instar larvae.They were also varied while different internal reference genes were used: the highest transcriptional levels were observed in tissues of midgut,silk gland and fat body while Actin3,GAPDH and 28S rRNA genes were used as the internal reference,respectively.Transcription of BmGSTd1 gene in various tissues could be induced by sodium fluoride applied onto mulberry leaves from the 2nd day of the 5th instar.And the expression varied in different tissues.The normalized data with the above 3 internal reference genes indicated that the expression of BmGSTd1 gene was the highest in mid-gut and fat body,suggesting their involvement in detoxification of sodium fluoride.It is thus concluded that,for detection of gene transcriptional levels,it is very important to choose adequate internal reference genes so as to ensure the reliability of the detection results." @default.
- W2373200616 created "2016-06-24" @default.
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- W2373200616 date "2010-01-01" @default.
- W2373200616 modified "2023-09-25" @default.
- W2373200616 title "Induced Expression and Quantitative Analysis of Bombyx mori Glutathione S-transferase Gene BmGSTd1" @default.
- W2373200616 hasPublicationYear "2010" @default.
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