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- W2373202904 abstract "Objective To establish a HPLC method for determination the plasma concentration of flucloxacillin in healthy volunteers. Methods Plasma samples were extracted by ethyl acetate,using carbamazepine as internal standard. The analysis was conducted with a Zorbax XDB-C8 column,acetonitrile -0.05 mol.L-1 monopotassium phosphate (28∶72) was served as mobile phase.The detecting wavelenth was at 225 nm.Results The calibration curve for flucloxacillin was linear in the range of 0.1 to 200.0 μg.mL-1(γ=0.9983,n=3) and the limit of detection was 0.1 μg.mL-1 . The absolute recoveries were 77.7%～83.0% at three different concentrations . The RSDs of intra-and inter-day were all less than 10.4% . Conclusion This method is suitable for the pharmacokinetic study of flucloxacillin in vivo." @default.
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- W2373202904 date "2008-01-01" @default.
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- W2373202904 title "Determination the plasma concentration of flucloxacillin in healthy volunteers by HPLC" @default.
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