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- W2373213744 abstract "Objective To isolate and identify adult adipose-derived mesenchymal stem cells which are able to be cultured in vitro and be differentiated from adult adipose tissue in order to provide materials for further research.Methods Using mechanical-collagenase digestion method to isolate adipose tissues from patients who had caesarean birth.The cells were cultured,passaged and purified by differential attachment technique.The passage 6 cells were used to identify surface antigen CD13 by flow cytometry and to identify surface antigen CD90 by immunohistochemistry antibody staining.Results In the adult adipose tissue there was a kind of fibroblast-shaped cells which could be stably cultured,passaged and differentiated into adipocytes in vitro.Compared with the blank control,both surface marker CD13 and CD90 were positive in those cells.Conclusion Cells that very simillar to adipose-derived mesenchymal stem cells can be successfully isolated and identified using that method.Therefore,it can be used as an effective method and to provide basic materials for further studies." @default.
- W2373213744 created "2016-06-24" @default.
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- W2373213744 date "2009-01-01" @default.
- W2373213744 modified "2023-09-23" @default.
- W2373213744 title "Isolation,cultivation and identification of adult adipose-derived stem cells" @default.
- W2373213744 hasPublicationYear "2009" @default.
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