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- W2373246478 abstract "To explore the immunogenicity of mouse neural stem cells (NSCs), thus help to solve the problem of immunologic rejection in NSCs transplantation.(1) Thirty C57BL/6 mice were assigned randomly into two groups, then the two groups were immuned by intraperitoneal injection respectively with BALB/c mice's NSCs or hepatic cells (as control). Afterwards one-way mixed lymphocyte culture was performed with BALB/c mice's NSCs or hepatic cells and immuned C57BL/6 mice's T lymphocytes. Immune response of T lymphocyte was detected by liquid scintillation counter. (2) The MHC-I and MHC-II molecules of BALB/c mice NSCs were stained with FITC- and PE-labeled antibody respectively. Then the expression of MHC-I and MHC-II were detected by flow cytometry.As shown by liquid scintillation counter, the cpm value of NSCs was 16592.8 +/- 2865.3, and that of the hepatic cells was 27815.0 +/- 2416.3 (P<0.01). About (87.55 +/- 3.65)% NSCs expressed neither MHC-I nor MHC-II, but there were only about (27.45 +/- 1.86)% hepatocytes showed both MHC-I and MYC-II negative (P<0.01).The mouse NSCs show weaker immunogenicity and their immunogenicity is significantly weaker than their hepatocytes." @default.
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- W2373246478 date "2010-04-01" @default.
- W2373246478 modified "2023-09-23" @default.
- W2373246478 title "[The immunogenicity of mouse neural stem cells]." @default.
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