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- W2373287777 abstract "Objective: To construct human a disintegrin and metalloprotease 9(ADAM9) gene into a eukaryotic expression vector PXJ40-HA and study the expression of ADAM9 gene in transfected 293T cells.Methods: ADAM9 gene was amplified from a vector PCR4-TOPO ordered from Open Biosystems and cloned into PMD18-T vector to construct a vector named PMD18-T-ADAM9.The PMD18-T-ADAM9 and the PXJ40-HA double digested by the same restrict enzymes,the recombinant eukaryotic expression vector PXJ40-HA-ADAM9 subsequently yielded.The expression of ADAM9 gene in tansfected 293T cells were identified by Western blot.Results: The vector PXJ40-HA-ADAM9 was constructed successfully and the expression of ADAM9 gene was detected by Western blot finally.Conclusion: ADAM9 gene is obtained as well as its recombinant eukaryotic expression plasmid is successfully constructed and expressed in 293T cells.The expression vector would be a useful material for the ADAM9 stable expression cell model and further funciton investigation." @default.
- W2373287777 created "2016-06-24" @default.
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- W2373287777 date "2011-01-01" @default.
- W2373287777 modified "2023-09-24" @default.
- W2373287777 title "Construction and eukaryotic expression of ADAM9 gene" @default.
- W2373287777 hasPublicationYear "2011" @default.
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