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- W2373323477 abstract "Objective To construct a high efficient interference plasmid for hper1(human period,gene),and to make a platform for study of function of hper1.Method Four pTER-hper1 interference plasmids according to hper1 sequence were constructed,and tested by sequencing.The 4 constructed plasmids were transferred into SH-SY5Y cells,and the SH-SY5Y cells were seduced by horse serum.The whole RNA extracted from the SH-SY5Y cells was used to test the effect of interference plasmids by RT-PCR.P-p44/42 MAPK was analyzed by western blot technology.Result RT-PCR showed that the expression of hper1 in SH-SY5Y cells transferred pTER-hper1-Ⅱwas decreased by 84.9%,which demonstrated that there was high interference efficiency of pTER-hper1-Ⅱinterference plasmid.Western blot technology showed that P-p44/42 MAPK in SHSY5Y cells was increased.Conclusion The pTER-hper1 interference plasmid is successfully constructed,which establishes a basis for studying the function of hper1.hper1 may be a negative controller of MAPK pathway." @default.
- W2373323477 created "2016-06-24" @default.
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- W2373323477 date "2006-01-01" @default.
- W2373323477 modified "2023-09-26" @default.
- W2373323477 title "Construction of the hper1 Interference Plasmid and Preliminary Study on Function of hper1." @default.
- W2373323477 hasPublicationYear "2006" @default.
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