Matches in SemOpenAlex for { <https://semopenalex.org/work/W2373397415> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2373397415 abstract "The purpose of this study was to clone heart-type fatty acid binding protein(H-FABP) gene cDNA of Xuhuai goat,and to explore its bioinformatics function and the possibility of preparation of transgenic animals among heterogeneous species.The subcelluar location H-FABP was detected by EGFP fusion protein and its expression was observed in vitro.Reverse transcription PCR(RT-PCR) technology was used to clone the H-FABP gene cDNA of Xuhuai goat,its biological information characteristics was analyzed by online software,then the expression vector pEGFP-H-FABP was constructed.The transfection of goat fibroblasts(GEF) was performed by Liposomes(LTX),and fluorescence was observed under inverted microscope after 48 h.The RT-PCR was conducted to detect mRNA expression of H-FABP in GEF.The pEGFP-H-FABP was injected into mouse testicular and its expression was detected at the level of DNA and protein.The complete CDS size of H-FABP was 402 bp,encoding 133 amino acids with GenBank accession number(AY466498.1).The H-FABP cDNA coding sequence was compared with the corresponding regions of human,chicken,brown rat,cow,wild boar,donkey and zebra fish,the similarity was 89%,76%,85%,84%,93%,91%,70%,respectively,amino acid sequence homology was 90%,79%,88%,97%,95%,94%,72%,respectively.The signal peptide was not found in H-FABP protein.The RT-PCR results showed the H-FABP mRNA expressed successfully in vitro.pEGFP-H-FABP was successfully constructed,and H-FABP mRNA was expressed.The H-FABP protein was localized in the cytoplasm which was in line with the result of online prediction.The gene can aslo be expressed in mice transiently and persistencely after intravenous and testicular injection.The H-FABP gene cDNA of Xuhuai goat was cloned successfully,and it was conservative during the evolutionary process,there was no signal peptide in protein.The H-FABP protein was located in the cytoplasm,and also could be expressed in mice successfully." @default.
- W2373397415 created "2016-06-24" @default.
- W2373397415 creator A5038816211 @default.
- W2373397415 date "2012-01-01" @default.
- W2373397415 modified "2023-09-26" @default.
- W2373397415 title "Gene Clone,Subcellular Localization of Expression Products of H-FABP and the Preparation of Transgenic Mice in Xuhuai Goat" @default.
- W2373397415 hasPublicationYear "2012" @default.
- W2373397415 type Work @default.
- W2373397415 sameAs 2373397415 @default.
- W2373397415 citedByCount "0" @default.
- W2373397415 crossrefType "journal-article" @default.
- W2373397415 hasAuthorship W2373397415A5038816211 @default.
- W2373397415 hasConcept C104317684 @default.
- W2373397415 hasConcept C105580179 @default.
- W2373397415 hasConcept C123894998 @default.
- W2373397415 hasConcept C150194340 @default.
- W2373397415 hasConcept C153911025 @default.
- W2373397415 hasConcept C187882448 @default.
- W2373397415 hasConcept C2776834966 @default.
- W2373397415 hasConcept C33161422 @default.
- W2373397415 hasConcept C40767141 @default.
- W2373397415 hasConcept C55493867 @default.
- W2373397415 hasConcept C86803240 @default.
- W2373397415 hasConceptScore W2373397415C104317684 @default.
- W2373397415 hasConceptScore W2373397415C105580179 @default.
- W2373397415 hasConceptScore W2373397415C123894998 @default.
- W2373397415 hasConceptScore W2373397415C150194340 @default.
- W2373397415 hasConceptScore W2373397415C153911025 @default.
- W2373397415 hasConceptScore W2373397415C187882448 @default.
- W2373397415 hasConceptScore W2373397415C2776834966 @default.
- W2373397415 hasConceptScore W2373397415C33161422 @default.
- W2373397415 hasConceptScore W2373397415C40767141 @default.
- W2373397415 hasConceptScore W2373397415C55493867 @default.
- W2373397415 hasConceptScore W2373397415C86803240 @default.
- W2373397415 hasLocation W23733974151 @default.
- W2373397415 hasOpenAccess W2373397415 @default.
- W2373397415 hasPrimaryLocation W23733974151 @default.
- W2373397415 hasRelatedWork W1416569895 @default.
- W2373397415 hasRelatedWork W1982806015 @default.
- W2373397415 hasRelatedWork W1986196587 @default.
- W2373397415 hasRelatedWork W2348467012 @default.
- W2373397415 hasRelatedWork W2348614087 @default.
- W2373397415 hasRelatedWork W2355905596 @default.
- W2373397415 hasRelatedWork W2358207002 @default.
- W2373397415 hasRelatedWork W2359886262 @default.
- W2373397415 hasRelatedWork W2366245834 @default.
- W2373397415 hasRelatedWork W2369139804 @default.
- W2373397415 hasRelatedWork W2371176156 @default.
- W2373397415 hasRelatedWork W2378589022 @default.
- W2373397415 hasRelatedWork W2383557450 @default.
- W2373397415 hasRelatedWork W2383947020 @default.
- W2373397415 hasRelatedWork W2384295054 @default.
- W2373397415 hasRelatedWork W2387684730 @default.
- W2373397415 hasRelatedWork W2389106485 @default.
- W2373397415 hasRelatedWork W2389110735 @default.
- W2373397415 hasRelatedWork W287688079 @default.
- W2373397415 hasRelatedWork W2967699766 @default.
- W2373397415 isParatext "false" @default.
- W2373397415 isRetracted "false" @default.
- W2373397415 magId "2373397415" @default.
- W2373397415 workType "article" @default.