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- W2373399456 abstract "In order to reveal the differentially-expressed genes involved in triterpenoid saponin biosynthesis of Siraitia grosvenorii,50 DAF(days after flowering)and 70 DAF fruits from different developmental stages were employed to construct subtractive cDNA library by suppression subtractive hybridization(SSH)in this study.Total 641 positive clones were selected randomly and sequenced from the forward-subtracted cDNA library(70 DAF as the tester and 50 DAF as the driver),and 622 high quality sequences were obtained.The rate of the recombination fraction was above 96%.The distribution of inserted fragments ranged from 101 bp and 934 bp,and the average fragment size was 500 bp.Followed by BLASTN and BLASTX for sequences dates,201 ESTs showed no significant matches to any other sequences in NCBI nucleotide sequence database,they were probably novel genes.The other 421 ESTs carried with remarkable identity to proteins with known function in the database,which fell into several functional categories including energy and secondary metabolism,transcription factors,ripening,senescence and pathogen-resistance.Further results indicated that the cDNA library quality were conformed to SSH library standard and could be used in further studies,also would provide reference for studying the differentially expressed genes,exploring molecular mechanism of triterpenoid saponin biosynthesis,and increasing productivity and quality of mogrosides in S.grosvenorii." @default.
- W2373399456 created "2016-06-24" @default.
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- W2373399456 date "2011-01-01" @default.
- W2373399456 modified "2023-09-25" @default.
- W2373399456 title "Construction of SSH-cDNA library from different developmental stages of Siraitia grosvenorii" @default.
- W2373399456 hasPublicationYear "2011" @default.
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