Matches in SemOpenAlex for { <https://semopenalex.org/work/W2373422902> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2373422902 abstract "AIM To clone cDNA of N terminal fragment of human bactericidal/permeability increasing protein(BPI), to construct the procaryotic and eucaryotic expression vectors and to express the cDNA in E.coli and CHO cells respectively. METHODS Total RNA was extracted from human polymorphonuclear neutrophils(PMN) and then the human BPI cDNA gene was amplified by nested RT PCR. The PCR product was cloned into pGEM T easy plasmid and the sequence was confirmed by restriction enzyme digestion and dideoxy mediated chain termination. Subsequently the specific BPI N terminal fragment was subcloned into pGEX 4T 1 plasmid and transformed into E.coli . In addition, the fragment was inserted into pcDNA3 plasmid and transformed into CHO cells with liposome transfection reagent. RESULTS A 726 bp fragment was acquired. The E.coli transformed with recombinant plasmid pGEX BPI was induced by IPTG. A 51 ku expected protein of GST BPI fusion protein was synthesized. The CHO cells transformed with pcDNA3 BPI were screened by G418, and the positive clone was obtained by using immunofluorescent assay. CONCLUSION N terminal of BPI was successfully expressed in E.coli and CHO." @default.
- W2373422902 created "2016-06-24" @default.
- W2373422902 creator A5077632892 @default.
- W2373422902 date "2000-01-01" @default.
- W2373422902 modified "2023-09-22" @default.
- W2373422902 title "Cloning and expression for cDNA of human bactericidal/permeability increasing protein N terminal fragment" @default.
- W2373422902 hasPublicationYear "2000" @default.
- W2373422902 type Work @default.
- W2373422902 sameAs 2373422902 @default.
- W2373422902 citedByCount "0" @default.
- W2373422902 crossrefType "journal-article" @default.
- W2373422902 hasAuthorship W2373422902A5077632892 @default.
- W2373422902 hasConcept C104317684 @default.
- W2373422902 hasConcept C121050878 @default.
- W2373422902 hasConcept C123894998 @default.
- W2373422902 hasConcept C153911025 @default.
- W2373422902 hasConcept C170344550 @default.
- W2373422902 hasConcept C185592680 @default.
- W2373422902 hasConcept C187882448 @default.
- W2373422902 hasConcept C19924922 @default.
- W2373422902 hasConcept C199360897 @default.
- W2373422902 hasConcept C22744801 @default.
- W2373422902 hasConcept C33161422 @default.
- W2373422902 hasConcept C40767141 @default.
- W2373422902 hasConcept C41008148 @default.
- W2373422902 hasConcept C54009773 @default.
- W2373422902 hasConcept C547475151 @default.
- W2373422902 hasConcept C55493867 @default.
- W2373422902 hasConcept C81089528 @default.
- W2373422902 hasConcept C86803240 @default.
- W2373422902 hasConceptScore W2373422902C104317684 @default.
- W2373422902 hasConceptScore W2373422902C121050878 @default.
- W2373422902 hasConceptScore W2373422902C123894998 @default.
- W2373422902 hasConceptScore W2373422902C153911025 @default.
- W2373422902 hasConceptScore W2373422902C170344550 @default.
- W2373422902 hasConceptScore W2373422902C185592680 @default.
- W2373422902 hasConceptScore W2373422902C187882448 @default.
- W2373422902 hasConceptScore W2373422902C19924922 @default.
- W2373422902 hasConceptScore W2373422902C199360897 @default.
- W2373422902 hasConceptScore W2373422902C22744801 @default.
- W2373422902 hasConceptScore W2373422902C33161422 @default.
- W2373422902 hasConceptScore W2373422902C40767141 @default.
- W2373422902 hasConceptScore W2373422902C41008148 @default.
- W2373422902 hasConceptScore W2373422902C54009773 @default.
- W2373422902 hasConceptScore W2373422902C547475151 @default.
- W2373422902 hasConceptScore W2373422902C55493867 @default.
- W2373422902 hasConceptScore W2373422902C81089528 @default.
- W2373422902 hasConceptScore W2373422902C86803240 @default.
- W2373422902 hasOpenAccess W2373422902 @default.
- W2373422902 hasRelatedWork W2347270363 @default.
- W2373422902 hasRelatedWork W2349162464 @default.
- W2373422902 hasRelatedWork W2357168565 @default.
- W2373422902 hasRelatedWork W2358377704 @default.
- W2373422902 hasRelatedWork W2358792118 @default.
- W2373422902 hasRelatedWork W2359752233 @default.
- W2373422902 hasRelatedWork W2360307462 @default.
- W2373422902 hasRelatedWork W2363390665 @default.
- W2373422902 hasRelatedWork W2363515636 @default.
- W2373422902 hasRelatedWork W2364227237 @default.
- W2373422902 hasRelatedWork W2367748249 @default.
- W2373422902 hasRelatedWork W2375749978 @default.
- W2373422902 hasRelatedWork W2389269354 @default.
- W2373422902 hasRelatedWork W2392821361 @default.
- W2373422902 hasRelatedWork W2398525087 @default.
- W2373422902 hasRelatedWork W2412645491 @default.
- W2373422902 hasRelatedWork W2419467415 @default.
- W2373422902 hasRelatedWork W2423933461 @default.
- W2373422902 hasRelatedWork W3139939178 @default.
- W2373422902 hasRelatedWork W90536018 @default.
- W2373422902 isParatext "false" @default.
- W2373422902 isRetracted "false" @default.
- W2373422902 magId "2373422902" @default.
- W2373422902 workType "article" @default.