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- W2373471371 abstract "Being sensitive to desiccation and low temperature,the recalcitrant seeds can not be stored with conventional methods. However,cryopreservation has been considered as the most effective means. As far as cryostorage of recalcitrant seeds is concerned,vitrification and two-step freezing have been applied in many studies. Unfortunately,there are some factors,including water content of seeds or embryonic axes,effects of cryopretectants in solutions,cooling rate and thawing,rehydration and after-thawing culture that influence succeeding in cryopreservation. To produce normal plants after cryopreservation of recalcitrant seeds or embryonic axes,a carefully defined sequence of manipulations is needed to be solved. Besides the mechanisms of desiccation tolerance and sensitivity to low temperatures,the freezing and antifreezing mechanisms of plant cells are also ascertained imminently in order to dig out the most effective long-term storage method through seed-gene bank." @default.
- W2373471371 created "2016-06-24" @default.
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- W2373471371 date "2007-01-01" @default.
- W2373471371 modified "2023-09-25" @default.
- W2373471371 title "Cryopreservation of recalcitrant seed germplasm" @default.
- W2373471371 hasPublicationYear "2007" @default.
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