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- W2373485339 abstract "Objective:To establish the primary culture of rabbit retinal Muller cells with the tissue suspending method. Methods:Rabbit retianl Muller cells were cultured in vitro with suspended constitution. Electron microscopy and immunocytochemical method were used to evaluate cells. Results:The cells attached to the wall of culture dishes while growing and showed large, flattened shape. Characteristic intermediate filament (8~10 nm) could be seen in the cytoplasm by transmission electron microscopy. The cultured cells showed positive reaction for glial fibriliary acid protein (GFAP) and S 100, and negative reaction for factor VIII related antigen with an immunocytochemical assay. Conclusion: The tissue suspending method is a stable and reliable method to obtain rabbit retinal Muller cells." @default.
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- W2373485339 date "2003-01-01" @default.
- W2373485339 modified "2023-09-25" @default.
- W2373485339 title "The Primary Cell Culture of Rabbit Retinal Müller Cell in Vitro" @default.
- W2373485339 hasPublicationYear "2003" @default.
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