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- W2373514114 abstract "Objective To research the feasibility of using polyhydroxybutyrate(PHB) and Pluronic F 127 together as the scaffolds for tissue engineered tissue.Method The chondrocytes isolated from cartilage in rabbit's rib were cultured and propagated to 3th generations in vitro.The 3th generations cells,which concentration was in 5×10 7/ml,were harvested and seeded into PHB directly or after being mixed with Pluronic F 127(at 4℃).Two kinds of different cells scaffold constructs which were PHB+cells(group A) and PHB+Pluronic F 127+cells(group B) respectively were incubated for one week in vitro,and were implanted subcutaneously into dorsum of autologous rabbits.Twelve weeks after the implantation,the specimens were collected and observe histologically to evaluate the characteristics of cartilage.All the data were analyzed afterwards.Result Specimens from two groups were subjected to histological observation.The results demonstrated that new cartilage was formed and secreated the cartilaginous matrix.There were a lot of neocartilages in supeficial and deep layers and the much cartilagenous matrix were secreated in goup B.In goup A,there were only a little of neocartilages in superficial layers,The cartilagenous matrix was less than goup B.Conclusion The cartilage formed using PHB coated Pluronic F 127 together as scaffolds is better than that using pure PHB,So component of PHB coated Pluronic F 127 is a better scaffold for tissue engineering." @default.
- W2373514114 created "2016-06-24" @default.
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- W2373514114 date "2002-01-01" @default.
- W2373514114 modified "2023-09-25" @default.
- W2373514114 title "The Feasibility Study of Tissue-engineered Tissue Using PHB and Pluronic F-127 Together As Scaffolds" @default.
- W2373514114 hasPublicationYear "2002" @default.
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