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- W2373519849 abstract "[Objective]To establish an in vitro model system for investigating ischemic postconditioning using renal tubular epithelial cell NRK-52E of rats,study the optimal scheme for ischemic postconditioning.[Methods]NRK-52E cells were randomly divided into 4 groups: Sham group,IRI group,HP group,and HP + group. In order to establish the in vitro model of renal ischemic postconditioning,the cells were treated with oxygen-glucose deprivation for 3 hours to simulate the? ischemia-reperfusion injury,and then the cells were exposed to the cycles of reperfusion condition for 10 minutes and ischemic condition for 10 minutes. The flow cytometry was used to test apoptosis,and the Western blotting technique was adopted to evaluate the level of cytochrome C.[Results] There was obvious apoptosis in NRK-52E cells subjected to ischemia-reperfusion for 24 hours(P 0. 05). The apoptosis in some ischemic postconditioning groups reduced significantly(P 0. 05),and the protection effect in HP + group was the best. The expression of cytochrome C increased significantly in NRK-52E cells subjected to ischemia-reperfusion for 24 hours(P 0. 05). In contrast,the expression of cytochrome C in ischemic postconditioning groups attenuated significantly,especially HP + group. [Conclusion]The in vitro model of ischemic postconditioning of NRK-52E is established successfully,and its mechanism? may be related to inhibition of apoptosis." @default.
- W2373519849 created "2016-06-24" @default.
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- W2373519849 date "2013-01-01" @default.
- W2373519849 modified "2023-09-22" @default.
- W2373519849 title "Establishment of an in vitro model system for investigating ischemic postconditioning using renal tubular epithelial cell of rats" @default.
- W2373519849 hasPublicationYear "2013" @default.
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