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- W2373529462 abstract "Objective To clone and express human cardiac troponin I cDNA,and to further investigate the method of obtaining the human cardiac troponin I (cTnI) at a high level.Methods Reverse transcription polymerase chain reaction based cloning method,cell transfection,and Western blot were used.Results The human cTnI encoding cDNA was successfully cloned and inserted into pcDNA3 eucaryotic expression vector.The expressed HA cTnI fusion protein was antigenic against anti HA and anti cTnI monoclonal antibodies.Conclusion The method provides a way for the eventual goal of obtaining a source of recombinant cTnI for diagnostic use." @default.
- W2373529462 created "2016-06-24" @default.
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- W2373529462 date "2000-01-01" @default.
- W2373529462 modified "2023-09-25" @default.
- W2373529462 title "Cloning and eucaryotic expression of the cDNA encoding human cardiac troponin I" @default.
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