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- W2373574357 abstract "Plant chloroplast Mg-chelatase,the first enzyme at the branch of chlorophyll biosynthesis pathway,catalyzes the incorporation of Mg2+ into protoporphyrin IX.Its three subunits,H,D and I,are products of nuclear coding genes,then imported into and function at the chloroplast.These subunits are not only involved in chlorophyll biosynthesis along with the enzyme,also exert other functions.Subunit H was found as an ABA receptor,and both H and D subunits were involved in the retrograde signaling from chloroplast to nucleus.Using a yeast two-hybrid system,we constructed a pGBKT7 bait vector containing pea Mg-chelatase D subunit cDNA and screened both a normalized nuclear-encoded and chloroplast-encoded cDNA library of pea leaves by co-transformation and mating method,respectively.In the total of 121 putative positive clones obtained,60 clones encode 21 chloroplast proteins,of which 19 were nuclear genes and 2 were chloroplast genes.The identified proteins were involved in different biological functions,such as chlorophyll biosynthesis,Calvin cycle,and regulation of protein translation and transcription in chloroplast,etc.By two hybrid re-cotransformation and GST pull-down assay,4 proteins were validated.Our results provided valuable clues for the functional studies of chloroplast Mg-chelatase D subunit." @default.
- W2373574357 created "2016-06-24" @default.
- W2373574357 creator A5032657316 @default.
- W2373574357 date "2011-01-01" @default.
- W2373574357 modified "2023-09-23" @default.
- W2373574357 title "Screening for Proteins Interacting with Mg-chelatase D Subunit in Pea Chloroplast Using Yeast Two-hybrid System" @default.
- W2373574357 hasPublicationYear "2011" @default.
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