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- W2373588974 abstract "Aqueous two - phase extraction (ATPE) was applied in extraction of cytochrome C (Cyt c) from pork heart. Poly (ethylene glycol) (PEG) (14 % , w/w) /(NH4 )2SO4 (12 %, w/w) aqueous two - phase system was made in crude Cyt c solution, Cyt c was found to be concentrated in the salt phase. After removing the salt, permutit was used to adsorb and elute Cyt c, and trichloroacetic acid (TCA) was added to precipitate Cyt c to obtain semifinished Cyt c product. Pure Cyt c was refined by using a weakly acidic cation exchange column. Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE), absorption spectral scan demonstrated that Cyt c product was obtained by this method was good in quality with higher productivity. Comparing to conventional methods, this technique possessed the characters of quick and simple in process with lower cost." @default.
- W2373588974 created "2016-06-24" @default.
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- W2373588974 date "2006-01-01" @default.
- W2373588974 modified "2023-09-24" @default.
- W2373588974 title "Application of Aqueous Two - phase Extraction to Fast Preparation of Cytochrome C from Pork Heart" @default.
- W2373588974 hasPublicationYear "2006" @default.
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