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- W2373613618 abstract "ES like cells of goat were isolated by the traditional method of isolation and cloning of primordial germ cells (PGCs) and the method of co culture of PGCs and the cells surrounding the gonadal anlage, from day 44 fetuses of healthy local white goat after natural estrus and mating. These ES like cells were cultured on different feeders. The results indicated that ES like cells were both derived from the traditional method and co culture in medium with cell growth factors. The ES like cells on primary homologous(goat) embryonic fibroblasts(PGEF) feeder grew better than those on primary mouse embryonic fibroblast(PMEF), which were observed for 4 5 passages and 3 passages respectively. However, only one ES like cell colony was derived by co culture in medium without cell growth factor and was lost after passage." @default.
- W2373613618 created "2016-06-24" @default.
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- W2373613618 date "2002-01-01" @default.
- W2373613618 modified "2023-09-25" @default.
- W2373613618 title "Isolation and Cloning of Goat ES-like Cells from PGCs" @default.
- W2373613618 hasPublicationYear "2002" @default.
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