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- W2373617783 abstract "An antiviral factor was detected in grass carp ( Ctenopharyngodon idellus ) leucocyte culture supernatant following induction with phytoagglutinin during October, 1997 to March, 1999 in Hangzhou City, Zhejiang Province, China. Biochemical study showed that the factor could not be dialyzed and sedimented at 10×10 4 g, exhibited stability to treatment of DNase, RNase and lipid solvent, but was sensitive to trypsin, glucosidase and NaIO 4. According to the above characteristics it should be a glycoprotein. Its inhibitory effect on virus replication was dependent on the process of cellular RNA and protein syntheses. It could inhibit different kinds of virus in the cells of grass carp, but failed to do so in human, mammalian, avian and shellfish cells. The result indicated the antiviral factor was specific to fish cells and viruses. Its biological characteristics were consistent with those of high vertebrate interferon. Furthermore, it was induced from the leucocytes by phytoagglutinin, sensitive to heat (56℃), pH=2 and 0.1% SDS. Its antigenic characteristic was different form that of interferon α/β induced by the virus, and its production could be promoted significantly by the phorbol ester and interleukin 2. These characteristics of the antiviral factor accorded with those of interferon gamma. The results revealed that the antiviral factor was an interferon gamma and there were different types of interferon in fish." @default.
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- W2373617783 date "2001-01-01" @default.
- W2373617783 modified "2023-09-25" @default.
- W2373617783 title "IN VITRO INDUCTION OF INTERFERON GAMMA FROM CTENOPHARYNGODON IDELLUSS LEUCOCYTES BY PHYTOAGGLUTININ" @default.
- W2373617783 hasPublicationYear "2001" @default.
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