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- W2373649223 abstract "During spermiogenesis, nuclear histones of somatic type are gradually replaced by newly synthesized spermatozoa-specific protamines in vertebrates. Protamine may play a role in transcriptional control. There is no experimental evidence to support it now. Using a very sensitive, new fluorescent method, the effect of protamine on transcription at 1- cell stage in mouse embryo was investigated. Anti-protamine antibody was injected into cytoplasm 6 hours after fertilization . The experiment shows that the fluorescent intensity in antibody-injected eggs differed from that in non-antibody-injected eggs,with a fluorescent intensity of 228% compared with the latter. The difference was statistically significant based on t′ test. In the experiment performed with BSA replacing protamine antibody, the difference between injected and non-injected eggs did not indicate statistical significance, which excluded the possibility that the difference might cause by nonspecific action of protein itself. In order to confirm the above observation, eggs were microinjected with both α-amanitin and protamine antibody in another experiment. Although protamine antibody was injected, the difference of fluorescent intensity between injected and non-injected eggs were abolished. All the results demonstrate that protamine plays an inhibitory role in the transcription of 1-cell stage fertilized eggs in the mouse." @default.
- W2373649223 created "2016-06-24" @default.
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- W2373649223 date "2002-01-01" @default.
- W2373649223 modified "2023-09-23" @default.
- W2373649223 title "The Effect of Protamine on the Transcription of 1-Cell Stage Fertilized Eggs in the Mouse" @default.
- W2373649223 hasPublicationYear "2002" @default.
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