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- W2373700713 abstract "To investigate the effect of Tanshinone ⅡA on the secretion and expression of VEGF and TGF-β1 in cultured human peritoneal mesothelial cells(HPMCs)induced by peritoneal dialysis fluid(PDF).The HPMCs cultured in vitro are used for experiment.After being incubated with DMEM medium for 24 hours,the cells were divided into five groups(DMEM medium as control,PDFgroup,100 umol/L Tanshinone II group,PDF containing 50umol/L and100 umol/L Tanshinone ⅡA as Tanshinone ⅡA 1 group and Tanshinone ⅡA2 group.Each group was supplemented with equal volume of DMEM culture medium containing 20%FCS.After 72 hours incubation the levels of VEGF and TGF-β1 in the supernatant and the mRNA of VEGF and TGF-β1 were detected.The activity of HPMCs was detected by Mitochondrial dehyrogenases actvity(MTT) in different concentrations of Tanshinone ⅡA.The rcesulte showed that the levels of VEGF and TGF-β1 in the supernatant were significant lower than those in PDF group.Markedly decreased levels of VEGF and TGF-β1 were found in Tanshinone ⅡA 1 group and Tanshinone ⅡA 2 group as compared with those in the PDF group.No significant difference was found between Tanshinone ⅡA 1 group and Tanshinone ⅡA2 group.Higher mRNAs of VEGF and TGF-β1 were in PDF group as compared with those in Tanshinone ⅡA and control groups.The upregulated secretion and mRNAs of VEGF and TGF-β1 induced by PDF can be inhibited by Tanshinone ⅡA in cultured HPMCs." @default.
- W2373700713 created "2016-06-24" @default.
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- W2373700713 date "2011-01-01" @default.
- W2373700713 modified "2023-09-27" @default.
- W2373700713 title "The Effect of Tanshinone IIA on the Secretion and Expression of VEGF and TGF-β1 in Cultured Human Peritoneal Mesothelial Cells" @default.
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