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- W2373722788 abstract "Agrobacterium tumefaciens-mediated transformation (ATMT) system is a significant technical method in the study of many filamentous fungi. We successfully transformed the Trichoderma viride LTR-2 by A. tumefaciens mediated method with an efficiency of 30-80 transformants per 10 5 conidia. PCR analysis showed that only the T-DNA containing foreign gene chi113 encoding chitinase from Bacillus subtilis was integrated into the genome, and the foreign gene chi113 remained stably in transformants. The transformation system mediated by Agrobacterium tumefaciens may prove to be a powerful tool for the filamentous fungi transformation and functional genomic study with its high transformation frequency, simplicity of T-DNA integration, and genetic stability of transformants." @default.
- W2373722788 created "2016-06-24" @default.
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- W2373722788 date "2005-01-01" @default.
- W2373722788 modified "2023-09-25" @default.
- W2373722788 title "Modification of Trichoderma viride by Agrobacterium tumefaciens-mediated transformation" @default.
- W2373722788 hasPublicationYear "2005" @default.
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