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- W2373722887 abstract "The specific primers were designed referring to the reported gene sequences (GenBank accession numberCD644775) of serine proteinase inhibitor first found from crayfish Procambarus clarkii. Serine proteinase inhibitor gene (PCI188) was amplified by reverse transcription polymerase chain reaction (RT-PCR) method using total RNA isolated from crayfish hemolymph as template. The amplified cDNA fragments were purified and inserted into pET-32a vector, then transformed into E.coli BL21 (DE3) and Rosetta. They induced by IPTG for the expression of recombinant protein. The protein was only expressed in Rosetta other than in BL21.The expressed fusion protein was identified by SDS-PAGE and Western-blotting. The results showed that 50 kD fusion protein was identical with the known protein coded by gene PCI188 of crayfish. Recombinant protein was purified and used to immunize the rabbit. Then phenoloxidase (PO) activities were measured by spectrophotometer after the immunized serum incubated with crayfish haemolymph. The results showed that immunized serum increased the PO activity of crayfish. This paper is the first to prove that the protein coded by gene PCI188 of crayfish can inhibit serine proteinase." @default.
- W2373722887 created "2016-06-24" @default.
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- W2373722887 date "2005-01-01" @default.
- W2373722887 modified "2023-09-28" @default.
- W2373722887 title "Cloning and expressing of a new serine proteinase inhibitor gene of crayfish and its activity analysis" @default.
- W2373722887 hasPublicationYear "2005" @default.
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