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- W2373725597 abstract "Objective: To evaluate the specificity and sensitivityof the 21/13 Alpha satellite probe which amplified by PCR.Explore and establish the clinical value of the probe and the method for prenatal diagnosis.Methods: In order to detect the specificity and sensitivity of the person 21/13 alpha satellite probe this was amplified by PCR,using the probe hybrid to medium-term lymphocyte chromosome.And to detect the specificity and sensitivity of the probe in prenatal diagnosis,using the probe hybrid to 50 cases of the uncultivated amniotic fluid cells,meanwhile for cell culture and karyotype analysis.Results: The results showed that the hybrid points in 21 and 13 of the centromere chromosome.The average detection rate for the normal group and the DOWNS group was 96%;Probe for amniotic fluid cells,the detection rate was 85.6%,and the accuracy rate was 100%.Conclusion: The 21/13 alpha satellite probe can detect the number of 21 or 13 chromosome of the amniotic fluid cell interphase nuclei for simple,rapid and accurate,Downs syndrome and Patau syndrome can be diagnosed by this probe." @default.
- W2373725597 created "2016-06-24" @default.
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- W2373725597 date "2011-01-01" @default.
- W2373725597 modified "2023-09-22" @default.
- W2373725597 title "Application of the 13/21 α satellite probe which was amplified by PCR for the study of prenatal diagnosis" @default.
- W2373725597 hasPublicationYear "2011" @default.
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