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- W2373726392 abstract "In this paper, SMART(switching mechanism at 5'end of RNA transcript)was used in theconstruction of cDNA library of postharvested banana fruit. Total RNA was isolated from banana fruit afterharvested for 2 days and cDNA was synthesized by using 15-25 round LD-PCR(Long-distance PCR). Fulllength of amplified ds-cDNA (Double-strained complement DNA) was obtained and used in the construction ofcDNA library afterward. The capacity of the library was measured to be 5×106 Pfu ml-1, with 93% recombinant. A cDNA was amplified by using PCR with this library diluted in 96-well-plate as templates. The sequence of this cDNA shows 83% similarity to Actin2 gene in the NCBI database (Accession No. 692696)." @default.
- W2373726392 created "2016-06-24" @default.
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- W2373726392 date "2005-01-01" @default.
- W2373726392 modified "2023-09-26" @default.
- W2373726392 title "The Construction of SMART cDNA Library from Banana Fruit and the Screening of Actin2 Gene by Using PCR Method" @default.
- W2373726392 hasPublicationYear "2005" @default.
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