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- W2373820208 abstract "Prokaryotic expression plasmid,pET30a-cpHSP70of C.plicata was constructed in this study.The construct was identified by endonuclease digestion and DNA sequencing and then transformed into the host bacteria BL21(DE3).1mmol·L-1 IPTG was used to induce the expression of the fusion protein.The expression product was verified by SDS-PAGE and Western blot.It was found that the molecular weight of new expressed protein was 74kD,which was similar to the expected molecular weight.The expression products increased with the increasing of induction time by IPTG.The optimal condition for protein expression was induction with 1mmol·L-1 IPTG at 37℃for 8h.Part of the expressed protein was soluble in supernatant fluid,while there was still some insoluble protein appeared in the inclusion bodies.The results of western-blot indicated that the protein levels of HSP70at 35℃ were increased rapidly in bloods,gills,muscles,hepatopancreas and mantles from C.plicata,compared to that of HSP70at 20℃." @default.
- W2373820208 created "2016-06-24" @default.
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- W2373820208 date "2014-01-01" @default.
- W2373820208 modified "2023-09-24" @default.
- W2373820208 title "Prokaryotic expression and preparation of polyclonal antibodie of heat shock protein 70 in Cristaria plicata" @default.
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