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- W2373931286 abstract "Objective To cultivate and identify the neurons transfected with recombinant adenovirus cardiotrophin-1(Adv-CT1).Methods Hippocampal neurons were isolated and cultured from new born Wistar rats and divided into two groups: Adv-CT1 and control group.The immunocytochemistry technique was used to test the neuron transfection efficiency.The trypanblau staining method was used to test the neuron mortality rate.Results In 12,24,36,48 and 60 hours after transfected with Adv-CT1,transfection rate of neurons was 43%,75%,90%,95% and 97%,respectively,and at the same times,the death rate of neurons was significantly lower in those transfected with Adv-CT1 than that in control group.Conclusions CT1 gene could be expressed steadily in neurons,in vitro,the ideal transfection rate was achieved at 48 hours.Transfected with Adv-CT1 could protect the neurons form damage." @default.
- W2373931286 created "2016-06-24" @default.
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- W2373931286 date "2010-01-01" @default.
- W2373931286 modified "2023-09-23" @default.
- W2373931286 title "Culture and identification of neurons transfected with recombinant adenovirus cardiotrophin-1" @default.
- W2373931286 hasPublicationYear "2010" @default.
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