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- W2373981320 abstract "Objective:To construct lentiviral vector of OT gene expression in Brandt's vole and detect the expression quantity and expressed area of OT gene.Methods:Total RNA extracted from brain tissue of Brandt's vole was amplified by RT-PCR for the cDNA fragments of OT gene and connected to two kinds of lentiviral vector,down stream of PGK or EF1A respectively.The two kinds of recombinant plasmid with OT gene labeling of luciferase,and eGFP marker respectivly,were transfected into 293T cells.The OT gene expression was detected in vivo by fluorescent imaging of luciferase or eGFP marker.At the same time,SYBR Green I real-time RT-PCR system was established to detect the copy number of OT gene in different samples.Results:The lentiviral vector labeled by LUC and EGFP was successfully constructed to express oxytocin gene of Brandt's vole,and real-time fluorescent PCR method was also established to detect the copy numbers of OT gene in transgenic cells.Conclusion:Oxytocin gene is a peculiar neurohypophysial hormone of mammal,associated with social cognition behaviors and social adaption behaviors." @default.
- W2373981320 created "2016-06-24" @default.
- W2373981320 creator A5065708102 @default.
- W2373981320 date "2013-01-01" @default.
- W2373981320 modified "2023-09-26" @default.
- W2373981320 title "Cloning of Brandt's vole oxytocin gene and its over-expression after cell transfection on lentiviral vector" @default.
- W2373981320 hasPublicationYear "2013" @default.
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