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- W2374017307 abstract "AIM To explore if nitrosoglutathione(GSNO) can activate rat liver microsomal glutathione transferases(mGST) in vitro via cysteine S nitrosylation. METHODS Partially purified mGST was incubated with GSNO in vitro, kinetic parameters of mGST were measured. N ethylmaleimide(NEM) reactivation and dithiothreitol(DTT) reversibility tests were performed, combined with the changes of free sulfhydryl group and S nitrosylated protein, to demonstrate the relevant mechanism. RESULTS Rat mGST was activated by GSNO in a concentration and time dependent manner, within the concentrations of 0.125-2 mmol·L -1 . NEM failed to reactivate the GSNO pretreated mGST, while DTT almost completely reversed the effect of GSNO. After incubated with GSNO, free sulfhydryl group in partially purified mGST significantly reduced, and S nitrosylated protein increased, both in a concentration dependent manner. CONCLUSION Rat liver microsomal glutathione transferases could be activated by GSNO, possibly via S nitrosylation of the single cysteine(Cys 49 ) in mGST." @default.
- W2374017307 created "2016-06-24" @default.
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- W2374017307 date "2003-01-01" @default.
- W2374017307 modified "2023-09-25" @default.
- W2374017307 title "Mechanism of the activation of rat liver microsomal glutathione transferases by nitrosoglutathione in vitro" @default.
- W2374017307 hasPublicationYear "2003" @default.
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