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- W2374020219 abstract "[Objective] To optimize proper methods for analyzing contents of five biogenic amines in catering waste. [Method]The method of detecting the contents of five biogenic amines in catering waste by RP-HPLC was developed. Samples were extracted by trichloroacetic acid,degreased with hexane,derived using benzoyl chloride and concentrated by nitrogen blowing for RP-HPLC testing. Waters RP18 column( 4. 6mm × 150 mm,3. 5μm) was used. The mobile phase was a gradient elution program with a binary mixture of methanol and water. The flow rate was 0. 8 ml/min,column temperature was 30 ℃ and UV detection wavelength was 254 nm. [Result]It was linear in the range of concentrations between 0. 50 and 50. 00 μg/ml( R2 0. 99). Detection limit of biogenic amines: putrescine,cadaverine as 0. 1μg/ml,spermine,histamine,tyramine was 0. 5μg/ml. The relative standard deviation( RSD) were less than 6%. The average recovery ranged from 67% to120% for all amines. [Conclusion] The results showed that the method has high sensitivity,repeatability and accuracy,suitable for catering waste in the qualitative and quantitative analysis of biogenic amines." @default.
- W2374020219 created "2016-06-24" @default.
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- W2374020219 date "2013-01-01" @default.
- W2374020219 modified "2023-09-22" @default.
- W2374020219 title "Determination of Biogenic Amines in Catering Waste by RP-HPLC" @default.
- W2374020219 hasPublicationYear "2013" @default.
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