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- W2374052178 abstract "Objective To construct the pEGFP-N1-ZIP2 expression vector,observe its expression in the human T lymphocyte cell line Jurkat-E6-1 and human mononuclear cell line THP-1,and detect whether expression of other zinc transporters changed when ZIP2 is over-expressed.Methods The target cDNA sequence of ZIP2 was obtained from human blood by RT-PCR.The target cDNA segment was ligated into eukaryote plasmid pEGFP-N1.The plasmids were checked by double restriction enzyme digestion and DNA sequence analysis.Jurkat-E6-1 and THP-1 cell lines were transiently transfected for 48 h,and expressions of ZIP2,ZIP1,ZIP6,ZIP8,ZIP10,ZnT1 and ZnT5 were detected by RT-PCR.Results Identification of pEGFP-N1-ZIP2 by double enzyme digestion and DNA sequence analysis showed that the length and direction of the target gene inserted into the recombinant plasmid were correct.After transfection of pEGFP-N1-ZIP2,ZIP2 over-expression was found in Jurkat-E6-1 and THP-1 cell lines.Expression of ZnT1 was significantly reduced in the transfected Jurkat-E6-1.However,on change was found in other zinc transporters in the transfected THP-1.Conclusion The eukaryotic expression plasmid pEGFP-N1-ZIP2 was successfully constructed.Jurkat and THP-1 cell lines were transfected with the plasmids.ZIP2 was up-regulated and ZnT1 was down-regulated in transfected Jurkat-E6-1,while no changes of other zinc transporter genes were found in transfected THP-1.Over-expression of ZIP2 has different effects on other zinc transporter genes in Jurkat-E6-1 and THP-1 cell lines." @default.
- W2374052178 created "2016-06-24" @default.
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- W2374052178 date "2012-01-01" @default.
- W2374052178 modified "2023-09-23" @default.
- W2374052178 title "Construction of the recombinant plasmid pEGFP-N1-ZIP2 and its effect on expression of other zinc transporters in human T lymphocyte and monocyte cell lines" @default.
- W2374052178 hasPublicationYear "2012" @default.
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