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- W2374052571 abstract "Objective To observe effects the of miR-200c on the proliferation of gastric carcinoma cells and to investigate whether miR-200c suppresses cell proliferation by targeting DNA-methyltransferase( DNMT3B). Methods The cell proliferation of human gastric carcinoma cells MGC-803 treated by miR-200c was detected by MTT. DNMT3B 3'UTR-luciferase vector was constructed,and luciferase reporter gene assay was employed to examine the effect of miR-200c on luciferase activity. MGC-803 cells were transfected with miR-200c mimics,and Western blotting was applied to detect the expression levels of DNMT3B protein. Results After transfection of miR-200c mimics for 24 h,48 h and 72 h respectively,MTT assay showed the OD of cell proliferation activity was 0. 31 ± 0. 01,0. 47 ± 0. 01 and 0. 53 ± 0. 02,while that in the control group was 0. 44 ± 0. 03,0. 62 ± 0. 01 and 0. 87 ± 0. 01; the differences were statistically significant( P 0. 05).Luciferase reporter vector system confirmed that DNMT3B was a target gene of miR-200c; Western blotting showed that the expression of DNMT3B protein was inhibited by miR-200c. Conclusion miR-200c suppresses cell proliferation by target regulating the expression of DNMT3B in gastric carcinoma." @default.
- W2374052571 created "2016-06-24" @default.
- W2374052571 creator A5014396820 @default.
- W2374052571 date "2013-01-01" @default.
- W2374052571 modified "2023-09-24" @default.
- W2374052571 title "Effects of miR-200c on proliferation of gastric carcinoma cells and the mechanism" @default.
- W2374052571 hasPublicationYear "2013" @default.
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