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- W2374059623 abstract "A novel silencer fragment(310 bp), which was recently discovered and identified by gel retardation assay and luciferase reporter gene expression analysis to locate between -2 132 bp and -1 822 bp upstream from the cap site of β globin gene, was subcloned {into a pUC T} vector. Then by using the DNA footprinting assays, it showed that there were two Hela cell nuclear protein binding sites in this fragment, one of them located at the sequence of -2 017～ -2 011 bp( i.e. “CTTCCGC”) and the other at -2 006～ -1 997 bp( i.e. “CACTTTATTT”). These two sequences were further sustained the site directed mutagenesis and accurately turned into the sequences of “CTTAAGC” and “CACTTAAGTT” respectively by two mutagenic primer in order to reconstruct mutated types of 310 bp fragment. They could be used to study the structure and function of the silencer as well as the mechanism of its regulation on gene transcription." @default.
- W2374059623 created "2016-06-24" @default.
- W2374059623 creator A5070758889 @default.
- W2374059623 date "2000-01-01" @default.
- W2374059623 modified "2023-09-25" @default.
- W2374059623 title "Subcloning and Site-directed Mutation of a Novel Silencer Fragment in 5′ Flanking Distal Region of Human β Globin Gene" @default.
- W2374059623 hasPublicationYear "2000" @default.
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