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- W2374065921 abstract "Objective:To detect prostate specific mem brane antigen m RNA (PSM- m RNA ) by a new sensitive nested reverse transcription polymerase chain reaction (RT- PCR ) assay with primers derived from the PSM c DNA sequence. Methods:Peripheral blood (10 ml) samples were collected from 4 0 patients with a biopsy- proven prostatic neoplasm s and 30 controls (15 benign prostatic hyperplasia,15 healthy young m ales) . PSM- m RNA was detected by nested RT- PCR with total RNA extracted from whole blood as the tem plate. L NCa P cells were used as positive control. Results:PSM- m RNA was detected in 2 4 blood samples from the 4 0 patients with prostatic neoplasm s (6 0 .0 % ) ,but there were no positive results in control group. In prostatic neoplasms group,PSM- m RNA was detected in the blood of 16 of 19patients with the evidence of metastasis (84 . 2 % ) ,while in the 2 1patients without m etastasis,only 8cases showed positive results (38. 1% ) . PSM- m RNA was also detected in the positive control,10 L NCa P cells in 10 m l blood showing positive result. Conclusion: PSM- m RNA can be a com plem ent indicator for prostate cancer. The positive rate of PSM- m RNA correlates markedly with the grade of prostatic neoplasm s,the status of metastases and the serum level of PSA. [" @default.
- W2374065921 created "2016-06-24" @default.
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- W2374065921 date "2002-01-01" @default.
- W2374065921 modified "2023-09-25" @default.
- W2374065921 title "Detection of prostate specific membrane antigen m RNA in peripheral blood by nested reverse transcription polymerase chain reaction" @default.
- W2374065921 hasPublicationYear "2002" @default.
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