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- W2374074166 abstract "The goat skin stem cells were isolated and cultured by two different methods of epidermal explant and dermal explant for the purpose of comparing the effeciency of the two methods on the cultivation and proliferation of skin stem cells in vitro.The resultant cells were observed and identified by cell growth curve,immnohistochemical staining and colony-forming efficiency.The results showed the skin stem cells can be separated by both methods;the skin stem cells from epidermal explant could be subcultured 11 times but the stem cells from dermal explant could be subcultured 17 times in vitro in the same culture condition;The positive expressions of K19、integrin-β1 and CFE of skin stem cells in the first passage by epidermal explant were(34.0±1.62)%,(37.5±2.12)%,(19.4±1.77)% respectively,and those in the first passage by dermal explant were(29.2±3.12)%,(33.0±1.12)%,(16.6±2.60)% respectively;The positive expressions of K19、integrin-β1 and CFE of skin stem cells in the third passage by epidermal explant were(46.4±1.82)%,(55.3±1.98)%,(25.3±1.08)% respectively,and those in the third passage by dermal explant were(53.7±1.17)%,(63.0±1.12)%,(30.9±2.16)% respectively;and the stem cells from dermal explant had higher efficiency in immnohistochemical staining,CFE and subculture ability were higher than those of epidermal explant when they were subcultured in vitro(P0.01)." @default.
- W2374074166 created "2016-06-24" @default.
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- W2374074166 date "2006-01-01" @default.
- W2374074166 modified "2023-09-23" @default.
- W2374074166 title "Study on the isolation of goat skin stem cells by explant" @default.
- W2374074166 hasPublicationYear "2006" @default.
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