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- W2374083080 abstract "This research adopt mCZB which added penicillin and streptomycin as control group, compared the impact of direct adding different consistence of Chinese medicine berberine(BR) to 2-cell in vitro development of mice and filtrated its optimal consistence, taking account of cells number of hatch embryos, observed the multiplication effect of Chinese medicine to cells number of embryo; Through transferring blastocysts which were cultured in vitro, validating the influence of Chinese medicine on effect of ET and litter development furthermore. The results as follows: Optimal level of BR was 0.10 μg /mL in vitro culture for 120 hours, the development ratio and cells number of HB of experiment groups(89.9%,83.7±9.10) were much better than those of control group(50.7%, 69.5±7.14)( P 0.01); Recipient gestation rate (66.7%)and living rate of away cote(55.8%)of experiment group were significant superior to control group(50.0%, 45.2%) (P 0.05).There weren't significant different in littering rate, mean weight of primiparous mice and mean weight of away cote mice between control group and experiment group(P 0.05). It is indicated that BR could promote development and cell multiplication of embryos, there weren't harmful impact to embryo implantation and firstborn mice." @default.
- W2374083080 created "2016-06-24" @default.
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- W2374083080 date "2006-01-01" @default.
- W2374083080 modified "2023-09-23" @default.
- W2374083080 title "Study on 2-cell Embryos in vitro Culture and Effect of Embryo Transfer of Mice by Adding a Chinese Medicine Berberine into Culture Medium" @default.
- W2374083080 hasPublicationYear "2006" @default.
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