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- W2374103496 abstract "To observe the expression of Foxp3 and IL-10 on CD4+CD25+ Treg induced by transforming growth factor-β1 (TGF-β1) in neonatal cord blood,cord blood mononuclear cells(CBMC) were stimulated with CD3 mAb and cultured in IL-2 containing medium with different concentrations of TGF-β1. The expressions of Foxp3 protein and Foxp3 gene mRNA in CD4+CD25+ Treg were detected by flow cytometry and RT-PCR assay,respectively,and the level of IL-10 in supernatant from cultured CBMC was detected by enzyme linked immunosorbent assay (ELISA). It was demonstrated that the expression of Foxp3 protein and mRNA in CD4+CD25+ Treg and IL-10 level in supernatant of cultured CBMC increased significantly in TGF-β1 treated group compared to control group. It is concluded that TGF-β1 up-regulates Foxp3 expression of the CD4+CD25+Treg and increases the secretion of IL-10 in cord blood in vitro." @default.
- W2374103496 created "2016-06-24" @default.
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- W2374103496 date "2010-01-01" @default.
- W2374103496 modified "2023-09-25" @default.
- W2374103496 title "Role of TGF-β1 in the expression of Foxp3 and IL-10 on CD4~+CD25~(high) Treg in neonatal cord blood" @default.
- W2374103496 hasPublicationYear "2010" @default.
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