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- W2374162334 abstract "A transcriptional cassette composed of human metallothinein promoter Ⅱ ( HuMT-Ⅱ ) ,HBsAg gene and SV40 early gene transcriptional processing signal was constructed.This cassette was inserted to a modified BPV-1 plasmid to form plasmid pdMTsAg-5. C127 mouse cells were transfected by this plasmid.Many tranformed foci were observed and isolated by the cloning ring method. AUSRIA test proved that out of 13 cell clones 12 can produced HBsAg. HBsAg yield studies were done on one of the clones.It was 292.6-525.8μg/l when harvested every 2 days and 200.9-369.0μg/1 when harvested every day.It can be harvested continuously for at least 60 days.The yield can be increased to 583-854.4μg/l when induced by heavy metalions.The harvested culture media were concentrated by ultrafiltration and purified by 2 cycles of density gradient ultracentrifugation. A HBsAg peak with Ausria I cpm 1.05×107 and buoyant density of 1.20 was obtained.Electronmicroscopy showed typical 22nm spherical particles." @default.
- W2374162334 created "2016-06-24" @default.
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- W2374162334 date "1986-01-01" @default.
- W2374162334 modified "2023-09-26" @default.
- W2374162334 title "EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN MAMMALIAN CELLS USING HUMAN METALLOTHIONEIN PROMOTER AND BOVINE PAPILLOMA VIRUS" @default.
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