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- W2374167916 abstract "Objective To construct prokaryotic expression vector of the Ts43 gene and express it in Escherichia coli. Methods The Ts43 gene was subcloned into pET-28a(+) and then expressed in E. coli DE3 inducing by IPTG. The fusion product was identified by SDS-PAGE and Western blot. Results The Ts43 gene was expressed in E. coli by inducing with IPTG. The peak of the target protein was presented at 4.5 h after adding 0.9 mmol/L IPTG. And a special reaction band to anti-Ts43 sera has been observed by Western blot. Conclusion The fusion protein of Ts43 gene expressed successfully in prokaryotic cells and it has a potential candidate for the recombinant subunit vaccines." @default.
- W2374167916 created "2016-06-24" @default.
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- W2374167916 date "2007-01-01" @default.
- W2374167916 modified "2023-09-27" @default.
- W2374167916 title "Cloning and prokaryotic expression of Trichinella spiralis Ts43 gene in Escherichia coli" @default.
- W2374167916 hasPublicationYear "2007" @default.
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