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- W2374175726 abstract "Objective To construct a mammalian co-expression plasmid of chemric IL-12-GPI cDNA and detect expression of fusion gene in RC-2 cells.Methods A mammalian co-expression plasmid of GPI-anchor-IL-12(human) were constructed by subcloning IL-12A (P35) gene and a fusion gene containing GPI-anchor signal sequence of hPLAP-1 and IL-12B (P40) in pBudCE4.1. Confocal laser scanning microscopy and flow cytometry were used to detect the expression of fusion protein in RC-2 cells.Results The mammalian co-expression plasmid was successfully constructed. Confocal laser microscopy analysis of the RC-2-GPI-IL-12 transfectants showed expression of IL-12 on the cell surface. PI-PLC-treated cells showed lower levels of cell surface expression of GPI-IL-12,indicting that IL-12 was anchored to the cell surfaces via a GPI-moiety.Conclusion The recombinant plasmid may provide foundation for further preparation of tumor vaccine." @default.
- W2374175726 created "2016-06-24" @default.
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- W2374175726 date "2009-01-01" @default.
- W2374175726 modified "2023-09-23" @default.
- W2374175726 title "Construction of mammalian co-expression plasmid of chemric IL-12-GPI cDNA" @default.
- W2374175726 hasPublicationYear "2009" @default.
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