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- W2374205486 abstract "Objective:Two plant expression vector of transcription factor CBF4 which control by promoter CaMV35s and inducible promoter CBF4P will be constructed.Method:First,constructed intermediate vectors SK-CBF4 and SK-CBF4P separately.Use EcoRⅠ cut CBF4 gene and CBF4P gene and connect to Bluescript M13(SK).Second,constructed plant expression vector p3301-CBF4 and p3301-CBF4P,which control by promoter CaMV35s and inducible promoter CBF4P.Use the multiple cloning site of SK and the same restriction sites of the plant expression vector p3301,connected the plant CBF4 and CBF4P on expression vector p3301.Result:Use PCR amplified recombinant plasmid p3301-CBF4 and p3301-CBF4P,obtained 670bp and the 1 200bp special fragment.Enzyme digestion also obtained the anticipated consistent DNA fragment.Conclusion:Two constructed expression vectors were correct,can be use in the plant heredity improvement and two promoters start efficiency research." @default.
- W2374205486 created "2016-06-24" @default.
- W2374205486 creator A5067126288 @default.
- W2374205486 date "2010-01-01" @default.
- W2374205486 modified "2023-09-28" @default.
- W2374205486 title "Construction of Plant Expression Vector Regulated Separately by Two CBF4 Gene Promoters" @default.
- W2374205486 hasPublicationYear "2010" @default.
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