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- W2374211321 abstract "Objective To analyze sequences of toxin-coregulated pilus (tcpA) gene of Vibrio cholerae (VC) strains isolated from China.Methods tcpA genes of 38 Vibrio cholerae strains were amplified by PCR, and sequenced.Results tcpA genes of 13 toxic strains of O139 serogroup VC and 15 toxic and epidemic strains of EVC were the same as that of EVC standard srtain N16961, and there were 4 mutated bases that divided strains into 5 sub-types. In 2 non-toxic and epidemic strains of EVC, one had the same tcpA gene as N16961, and another had new tcpA gene type. tcpA genes of 6 non-toxic and non-epidemic strains of EVC were divided into 2 new types. tcpA genes of 2 non-toxic and non-O1/non-O139 serogroup VC strains were 2 new types.The identities and divergences of 4 new tcpA gene types and 4 types found by others were 63.8%~84.6% and 17.8%~44.6%,those of 8 TcpA types were 60.6%~91.7% and 8.8%~45.8%.The Vibrio cholerae pathogenicity islands (VPI) of 4 new tcpA types were complete, but there were variable genes in VPIs.Conclusion Toxic VC O139 serogroup strains could be originated from toxic EVC strains, and differentiated more. Four new tcpA genes were found in non-toxic VC strains." @default.
- W2374211321 created "2016-06-24" @default.
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- W2374211321 date "2006-01-01" @default.
- W2374211321 modified "2023-09-27" @default.
- W2374211321 title "Sequence analysis of toxin-coregulated pilus gene of Vibrio cholerae strains isolated from China" @default.
- W2374211321 hasPublicationYear "2006" @default.
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