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- W2374219956 abstract "The bioanalyzer represents a new generation of capillary electrophoresis instruments for the plasma DNA separation based on microfluidics. DNA fragments of HIV-1 prepared by restriction display technique were analyzed with Agilent 2100 Bioanalyzer. The whole cDNA of HIV subtype B U26942 was cleavaged into fragments by restriction endonuclease Sau 3A I. The fragments were ligated with adapters and amplified by PCR with universal primer. Agilent 2100 Bioanalyzer and conventional agarose gel electrophoresis were then employed to examine the PCR products respectively. The comparison from two methods showed that Agilent 2100 Bioanalyzer could provide more accurate, and semi-quantitative information of HIV fragments than agarose gel electrophoresis." @default.
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- W2374219956 date "2005-01-01" @default.
- W2374219956 modified "2023-09-24" @default.
- W2374219956 title "Applying agilent 2100 bioanalyzer to analyze restriction displayed HIV DNA fragments" @default.
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