FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374253574> ?p ?o ?g. }

• W2374253574 endingPage "7338" @default.
• W2374253574 startingPage "7338" @default.
• W2374253574 abstract "Currently, there are no definitive immunomarkers for epithelial stem cells (corneal and conjunctival) or their poorly understood niche microenvironment. The H2B-GFP/K5tTA mouse enables visualization of label-retaining cells (LRCs), which exhibit the functional marker of stem cell quiescence. We used immunofluorescence tomography to evaluate putative stem cell markers and LRCs of the mouse ocular surface.H2B-GFP/K5tTA mice were pulsed for 56 days and then chased with doxycycline to label LRCs. Limbus and eyelid tissue was 3-dimensionally (3-D) reconstructed using immunofluorescence tomography to identify and characterize LRCs using the putative stem cell markers sox9, keratin 19, lrig1, blimp1, and abcb5.After 28 days of chase, LRCs were localized to the entire limbus epithelium and, infrequently, the anterior limbal stroma. Label-retaining cells comprised 3% of limbal epithelial cells after 56 days of chase. Conjunctival LRCs were localized to the fornix and comprised 4% of the total fornix epithelial cells. No stem cell immunomarker was specific for ocular surface LRCs; however, blimp1 enriched for limbal basal epithelial cells and 100% of green fluorescent protein-positive (GFP+) cells at the limbus and fornix were found to be lrig1-positive.Label-retaining cells represent a larger population of the mouse limbus than previously thought. They decrease in number with increased doxycycline chase, suggesting that LRC populations with different cell cycle lengths exist at the limbus. We conclude that current immunomarkers are unable to colocalize with the functional marker of epithelial stem cell quiescence; however, blimp1 may enrich for limbal epithelial basal cells." @default.
• W2374253574 created "2016-06-24" @default.
• W2374253574 creator A5023829373 @default.
• W2374253574 creator A5041670457 @default.
• W2374253574 creator A5050140915 @default.
• W2374253574 creator A5052807762 @default.
• W2374253574 creator A5057180220 @default.
• W2374253574 creator A5090570484 @default.
• W2374253574 date "2015-11-11" @default.
• W2374253574 modified "2023-10-17" @default.
• W2374253574 title "Immunofluorescence Tomography of Mouse Ocular Surface Epithelial Stem Cells and Their Niche Microenvironment" @default.
• W2374253574 cites W1248078671 @default.
• W2374253574 cites W1515583925 @default.
• W2374253574 cites W178514072 @default.
• W2374253574 cites W186621308 @default.
• W2374253574 cites W193927929 @default.
• W2374253574 cites W1967479683 @default.
• W2374253574 cites W1977959025 @default.
• W2374253574 cites W1982347881 @default.
• W2374253574 cites W1984996991 @default.
• W2374253574 cites W1998479150 @default.
• W2374253574 cites W2002935341 @default.
• W2374253574 cites W2016835212 @default.
• W2374253574 cites W2032155043 @default.
• W2374253574 cites W2039396493 @default.
• W2374253574 cites W2042718792 @default.
• W2374253574 cites W2054721944 @default.
• W2374253574 cites W2057514048 @default.
• W2374253574 cites W2057993832 @default.
• W2374253574 cites W2059168179 @default.
• W2374253574 cites W2063472373 @default.
• W2374253574 cites W2071595764 @default.
• W2374253574 cites W2081394394 @default.
• W2374253574 cites W2093559895 @default.
• W2374253574 cites W2097304851 @default.
• W2374253574 cites W2102699949 @default.
• W2374253574 cites W2104205689 @default.
• W2374253574 cites W2105409819 @default.
• W2374253574 cites W2119226652 @default.
• W2374253574 cites W2121062346 @default.
• W2374253574 cites W2126917358 @default.
• W2374253574 cites W2127292451 @default.
• W2374253574 cites W2127823194 @default.
• W2374253574 cites W2128078747 @default.
• W2374253574 cites W2140765527 @default.
• W2374253574 cites W2161092951 @default.
• W2374253574 cites W85037328 @default.
• W2374253574 cites W98873111 @default.
• W2374253574 doi "https://doi.org/10.1167/iovs.15-18038" @default.
• W2374253574 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4642609" @default.
• W2374253574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26559480" @default.
• W2374253574 hasPublicationYear "2015" @default.
• W2374253574 type Work @default.
• W2374253574 sameAs 2374253574 @default.
• W2374253574 citedByCount "27" @default.
• W2374253574 countsByYear W23742535742016 @default.
• W2374253574 countsByYear W23742535742017 @default.
• W2374253574 countsByYear W23742535742018 @default.
• W2374253574 countsByYear W23742535742019 @default.
• W2374253574 countsByYear W23742535742020 @default.
• W2374253574 countsByYear W23742535742021 @default.
• W2374253574 countsByYear W23742535742022 @default.
• W2374253574 countsByYear W23742535742023 @default.
• W2374253574 crossrefType "journal-article" @default.
• W2374253574 hasAuthorship W2374253574A5023829373 @default.
• W2374253574 hasAuthorship W2374253574A5041670457 @default.
• W2374253574 hasAuthorship W2374253574A5050140915 @default.
• W2374253574 hasAuthorship W2374253574A5052807762 @default.
• W2374253574 hasAuthorship W2374253574A5057180220 @default.
• W2374253574 hasAuthorship W2374253574A5090570484 @default.
• W2374253574 hasBestOaLocation W23742535741 @default.
• W2374253574 hasConcept C136302470 @default.
• W2374253574 hasConcept C142724271 @default.
• W2374253574 hasConcept C169760540 @default.
• W2374253574 hasConcept C2776882836 @default.
• W2374253574 hasConcept C2778155685 @default.
• W2374253574 hasConcept C2779257441 @default.
• W2374253574 hasConcept C28328180 @default.
• W2374253574 hasConcept C2908647359 @default.
• W2374253574 hasConcept C71924100 @default.
• W2374253574 hasConcept C86803240 @default.
• W2374253574 hasConcept C95444343 @default.
• W2374253574 hasConcept C99454951 @default.
• W2374253574 hasConceptScore W2374253574C136302470 @default.
• W2374253574 hasConceptScore W2374253574C142724271 @default.
• W2374253574 hasConceptScore W2374253574C169760540 @default.
• W2374253574 hasConceptScore W2374253574C2776882836 @default.
• W2374253574 hasConceptScore W2374253574C2778155685 @default.
• W2374253574 hasConceptScore W2374253574C2779257441 @default.
• W2374253574 hasConceptScore W2374253574C28328180 @default.
• W2374253574 hasConceptScore W2374253574C2908647359 @default.
• W2374253574 hasConceptScore W2374253574C71924100 @default.
• W2374253574 hasConceptScore W2374253574C86803240 @default.
• W2374253574 hasConceptScore W2374253574C95444343 @default.
• W2374253574 hasConceptScore W2374253574C99454951 @default.
• W2374253574 hasIssue "12" @default.
• W2374253574 hasLocation W23742535741 @default.
• W2374253574 hasLocation W23742535742 @default.

• next